Fig. 2. MI arrest in the ovaries of stimulated animals. (Left column) Oocytes spawned into SW at 45, 105 and 165 minutes after the injection of 1-MA into the body cavity of the animal were immediately collected and treated with extraction buffer, followed by fixation and staining with DAPI and an anti-tubulin antibody to visualize chromosomes (blue) and tubulin (green). (Right column) Oocytes incubated in normal SW for 30 minutes after spawning were similarly treated and stained at 75, 135, and 195 minutes. Representative figures are shown. (Top row) Prometaphase at 45 minutes and anaphase at 75 minutes. (Middle row) Metaphase at 105 minutes and anaphase at 135 minutes. (Bottom row) Metaphase at 165 minutes and anaphase at 195 minutes. Scale bar: 2 µm.

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