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Fig. 4. Inhibition of polar body formation in zero-Na+ artificial SW after 1-MA addition. GVBD occurred 20 minutes after 1-MA addition both in normal SW and zero-Na+ artificial SW. In normal SW, the first and second polar body (arrowheads) were extruded 70-90 minutes and 100-120 minutes after 1-MA addition, respectively. In zero-Na+ artificial SW, polar bodies were not extruded.





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