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Fig. 4. SpPks and SpFmo1 antisense morpholino oligonucleotide injected embryos. (A-C) Aboral view of prism stage embryos injected with 200 µM antisense morpholino oligonucleotide against the SpPks (B) and the SpFmo1 (C) genes and with a 200 µM random sequence morpholino oligonucleotide (A). The embryos injected with the SpPks and SpFmo1 morpholinos did not show accumulation of the echinochrome pigment. (D-F) Whole mount in situ hybridization with antisense RNA DIG-labeled probe for SpSult on prism stage embryos injected with a random sequence morpholino oligonucleotide (D), or with the SpPks (E) or the SpFmo (F) morpholino oligonucleotides. (E,F) Pigment cells are present in embryos lacking SpPKS or SpFMO.





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