Fig. 2. Quantitative analysis of precursor subtypes in wild-type and reeler mice throughout neurogenesis. The pie charts depict the quantitative co-localization analysis of precursor cells immunoreactive for Rc2, Blbp and Glast in acutely dissociated cells of wild-type and reeler cortex at E12, E14 and E16. The analysis was performed as described in detail in Hartfuss et al. (Hartfuss et al., 2001). Briefly, triple immunostainings were performed in different combinations of Rc2, anti-Glast, anti-Blbp and anti-Ki67 to detect all dividing cells as described in the above reference. Note that the number of Blbp-positive precursors is severely reduced in the reeler compared with wild-type cortex. [Number of cells analysed: wild-type, n(E12)=311, n(E14)=1821, n(E16)=302; reeler, n(E12)=307, n(E14)=1853, n(E16)=295.]

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