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Fig. 8. Effect of LPP3 in Wnt target gene expression. Expression analysis
of Wnt target genes during EB differentiation. Semi-quantitative RT-PCR
analysis of markers at the indicated days in culture. The loading control was
the Hprt gene. Bmp4 did not show significant differences in
expression during EB differentiation. In contrast, while brachyury
expression (a direct transcriptional target of the Wnt signaling pathway)
decreased after 6 days in culture in heterozygous EBs, its expression was
increased and prolonged in LPP3-/- EBs.
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