DEV00671 Supplemental Figures

Files in this Data Supplement:

• Supplemental Fig S1 - Ten-somite-stage fgf8^-;fgf24^MO embryos do not have mesodermal precursors in the tail bud. Expression of Ntl (brown) and spt (purple) in 10 somite-stage wild-type (A), fgf8 mutant (B) and fgf8^-;fgf24^MO (C) embryos reveals that fgf8^-;fgf24^MO embryos do not have mesodermal precursors that are located in the tail bud (white asterisks) and presomitic mesoderm (arrows) in wild type (A) and fgf8 mutants (B). In addition, analysis of these markers reveals that at this stage the tail buds of fgf8 mutant embryos (B) contain significantly fewer presomitic mesoderm precursors (spt expressing cells) in comparison with wild-type embryos (A). Scale bar: 50 mm.

• Supplemental Fig S2 - fgf24 does not appear to genetically interacts with either ntl or spt. Markers used for in situ hybridization and immunohistochemistry in A-C and G-H as well as identifiers (e.g. arrows) are as described for Fig. 4A. Representative pictures of stained 12-somite-stage embryos (A-C,G-H) and live 24 hpf embryos (D-F,J-L) are shown. (A,D) Wild type, (B,E) ntl mutant and (C,F) ntl mutant injected with fgf24 mopholino oligo. (G,J) Wild type, (H,K) spt mutant and (I,L) spt mutant injected with fgf24 mopholino oligo. Scale bars: in A, 50 mm for A-C; in G 50 mm for G-I.