Fig. 1. fgf24 is a member of the fgf8/17/18 subfamily and is expressed during gastrulation. (A) Sequence comparison of the predicted amino acid sequences of zebrafish (Dr) Fgf24, Fgf18, Fgf8 and Fgf17, with human (Hs) FGF18, FGF8 and FGF17. Periods indicate identical residues; dashes indicate introduced gaps; arrows indicate exon boundaries. Only partial sequence for zebrafish Fgf18 is shown as the 5' end of the gene has not been identified. (B) Phylogenetic tree comparing the relatedness of zebrafish Fgf18 and Fgf24 with other members of the Fgf8 subfamily. Zebrafish Fgf10 is distantly related to the Fgf8 subfamily, and was included as an outgroup. Numbers indicate bootstrap support for the nodes. (C) fgf18 and fgf24 map to LG 14. Map position of fgf18 and fgf24, as determined by screening the T51 radiation hybrid panel is shown relative to representative zmarkers (left) and ESTs (right, listed by GenBank Accession Numbers). The entire linkage group is not shown. Zebrafish genes that are closely linked to zebrafish fgf18 have human homologs that are closely linked to human FGF18. (D) Temporal expression profiles for zebrafish fgf18 and fgf24 in comparison with fgf8 as determined by RT-PCR. The zebrafish ornithinedecarboxylase (odc) gene was used as an internal control (see Draper et al., 2001). cR, centiRay; MWM, molecular weight marker; hpf, hours post fertilization.

Return to article