Fig. 2. Disorganization of the Bergmann glial network in young treated MBP-TK mice. (A-D) Immunofluorescence analysis of cerebellar sections from treated 6-day-old (A,B) or 21-day-old (C,D) wild-type (A,C) and MBP-TK (B,D) mice. Astrocytes were identified using specific antibodies directed against brain lipid binding protein (BLBP; A,B) and glial fibrillary acidic protein (GFAP; C,D). In the wild-type, the BLBP-positive soma of Bergmann glial cells (A; arrowheads) are located at the level of the Purkinje cell layer and their radial fibers extend across the ML toward the pial surface (C). In MBP-TK, Bergmann glial cells are dispersed throughout the folia (B) and their radial scaffold is strongly perturbed (D). Scale bars: 75 µm (A,B); 12 µm (C,D).

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