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Fig. 6. Inhibition of Ihh signalling reduces proliferation in the germinal region at e14. (A) control Q/QJ cultures cultured for 16 hours and labelled with BrdU. Bracket demarcates the germinal region, black arrowhead indicates proliferating cells. (B) Ihh expression in an adjacent section. (C) ptc2 is expressed in the germinal region (white arrowhead). (D-F) Hh blocking-antibody treated contralateral side. (D) The reduction in number of BrdU labelled cells is indicated by the black arrowhead. (E) An adjacent section showing unaffected Ihh expression. (F) An adjacent section showing downregulation of ptc2 expression in the germinal region (arrowhead). Scale bars: 100 µm.





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