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Fig. 5. Reduced size of efferent synapses in the absence of Bdnf and TrkB
signalling. Cochleae of adult TrkBshc/+,
TrkBshc/-, Bdnf+/+ and
Bdnf-/- mice at P19 were isolated, sectioned, and
double-immunostained with anti-synaptophysin and anti-SK2 antibodies as
described under Materials and methods. (A) Representative micrographs
illustrating synaptophysin (Syn; green) and SK2 (red) staining at the outer
hair cell (OHC) level of the basal cochlear turn in mice with the indicated
genotypes. Note the severe reduction of synaptophysin-positive boutons and of
SK2 staining opposite the synaptic bouton in
TrkBshc/- and Bdnf-/- mutant
mice compared with their respective controls. (B) Higher magnification view of
a representative OHC from A. Area outlined by dotted line represents the
position of the OHC body. Scale bars: 10 µm in A; 5 µm in B.
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