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Fig. 6. Expression of Bdnf, Nt3 and TrkB mRNA in the spiral ganglion of adult rats. (A) TrkB, Bdnf and Nt3 riboprobes specifically hybridise with spiral ganglia of the midbasal cochlea turn of P35 rat cochlea. Note the expression of Nt3 in the IHCs (inset). (B) The expression of Bdnf, Nt3 and TrkB was analysed in cochlear neurones along the tonotopic axis at the indicated levels (apical/medial/midbasal/basal) in rats. Bdnf and TrkB are expressed with increasing levels towards the basal part of the cochlea, whereas Nt3 mRNA shows an opposite gradient of expression, with the highest amounts of mRNA detected in the apex. SG, spiral ganglion. Scale bars: 10 µm in inset in A; 20 µm in B.





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