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Fig. 2. Brn1 is expressed in the developing loop of Henle (HL) and distal convoluted tubule (DCT) during nephrogenesis. Brn1 immunostaining (A,C,E,G,H) and schematic drawings of the key stages of nephron development (B,D,F,I). Brn1 immunoreactivity is present in sections of the renal vesicle (RV; A,B); within the prospective HL, the DCT and the macula densa of nascent S-shaped bodies (C,D); within the elongating HL (E,F); and within the thick ascending limb (TAL), macula densa (MD) and DCT (G-I) of the newborn kidney. Brn1- positive region of the nephron are indicated by a red outline in the schematic drawings (B,D,F,I). RV, renal vesicle; BC, Bowman's capsule; PCT, proximal convoluted tubule; CNT, connecting tubule; tDL, thin descending limb. Scale bar: 20 µm. (B,D,F,I) Modified, with permission, from Fischer et al. (Fischer et al., 1995).





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