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Fig. 6. Brn1 gene dosage effects on gene expression levels in Brn1 mutant
kidneys. We performed an RNase protection assay (RPA) using total RNA from
newborn and adult kidneys. The expression of Umod, Ptger3, Nkcc2 and
Kcnj1 (encoding apical K+ channel) were nearly
undetectable in the kidneys of newborn Brn1-/- mice. The
mRNA level of epidermal growth factor (Egf) in the
Brn1-/- kidney was reduced to 
20% to that of the wild
type in newborn. The mRNA levels of the basolateral TAL Cl- channel
(Clcnk1l) and its ß-subunit (Bsnd) in the
Brn1-/- kidney were reduced to about 30% of wild-type
levels in newborn. The mRNA levels of Nkcc2, Bsnd and Kcnj1,
indispensable regulators of Na+ reabsorption, were significantly
reduced in Brn1+/- kidneys in comparison with the levels
of Brn1+/+ kidney in both newborn and adult kidneys. The
mRNA levels of Umod and Ptger3 in
Brn1+/- kidneys were also significantly reduced in
comparison with Brn1+/+ kidneys. The mean values and
s.e.m. of the ratios of the mutant to the wild-type signals are displayed at
the right of the protected band patterns (n=3-4). All data were
normalized to the Gapd signal prior to statistical analysis.
*P<0.05, **P<0.001 compared with
Brn1+/+ kidney (ANOVA).
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