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Fig. 6. Comparison of E(spl)m7VP16 with E(spl)m7KNEQ-VP16 in the activation of EE4-lacZ and Ac expression. (A-F) X-gal staining of EE4-lacZ wing disks. (G-I) immunostaining for Ac protein. (A,G) Wild type. (B,H) UAS-E(spl)m7VP16; pnr-GAL4. (C,I) UAS-E(spl)m7KNEQ-VP16; pnr-GAL4. Reporter activation at the SC (white arrowhead) and DC (black arrowhead) proneural clusters is seen at equally high levels in B and C. (D) UAS-sc; pnr-GAL4. (E) UAS-sc UAS-E(spl)m7VP16; pnr-GAL4. F: UAS-sc UAS-E(spl)m7KNEQ-VP16; pnr-GAL4. X-gal development in D-F is at equivalent levels, as judged by the wing-margin proneural clusters, where pnr-Gal4 is not expressed. Note the increased transcriptional response of the EE4-lacZ reporter in E and F compared with D.





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