Fig. 6. Partial rescue of polytene puffing pattern by individual EcR isoforms. Each panel represents animals containing the Sgs3 driver and responders for EcR-F645A and for a wild-type EcR of the indicated isoform. Salivary glands were isolated from post-wandering larvae (black bars) and white prepupae (gray bars), and their developmental stage was confirmed by salivary gland duct morphology (Zhimulev and Belyaeva, 1999). The glands were squashed and puff stages of the chromosomes determined. White extensions of the bars indicate animals selected as white prepupae in which the puffing pattern showed minor deviations from the indicated puff stage. ? indicates animals with a puffing pattern too aberrant to permit assignment of a puff stage. Salivary glands from wild-type post-wandering larvae have PS7-9 polytene chromosomes, and those from wild-type white prepupae have PS10-11 chromosomes.

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