Fig. 2. Characterization of phol mutants. (A) The thick line indicates genomic DNA. The thin line indicates the extent of the phol transcription unit. The arrows indicate the start and direction of transcription of phol and the flanking transcription unit, CG3348. The transcription start sites are the first nucleotides of the ESTs from the Drosophila genome project. The shaded boxes indicate the extent of DNA deleted in the phol mutants. The approximate location of the start of the coding region (ATG), the conserved spacer region (S), and the zinc finger (zf) region are shown. (B,C) Embryos stained with DAPI and for the presence of the sperm tail (Karr, 1991). The embryo in B is from a wild-type mother and the embryo in C is from a phol mutant mother. The sperm tail is evident in both embryos (white arrows), but DAPI staining of nuclear DNA is evident only in the wild-type embryo (bright dots in centre of embryo).

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