Fig. 3. Examples of clones generated by fate-restricted neural crest cells. (A,B) Images of the same field of cells in a differentiated (108 hour) culture. This clone was derived from a single TrkC-expressing neural crest cell labeled during the 13-16 hour time period. The four members of the clone were identified by fluorescent lineage dye (arrows, A) and were found to be neurons by 16A11 immunoreactivity (arrows, B). (C,D) A two-cell clone in a differentiated culture derived from a C-Kit-expressing neural crest cell labeled during the 13-16 hour period. The two members of the clone (arrowheads, C) were melanocytes, as they contained melanin granules (arrowheads, D, phase-contrast). Scale bars: 25 µm in B; 12 µm in D.

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