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Fig. 3. Rescue of EnR-Dlx3hd activity by full-length Dlx3. A constant amount
ß-galactosidase and 50 pg of EnR-Dlx3hd mRNAs were injected unilaterally
as in Fig. 2 along with
increasing doses of mRNA encoding full-length Dlx3 (Dlx3 FL). (A-C) Embryos
stained for Xsox2 show the typical expansion of the neural plate
obtained with EnR-Dlx3hd (A), the rescue achieved in combination with Dlx3 FL
(B), and the overexpression phenotype typical of Dlx3 FL-injected embryos (C).
(D) Incidence of each of the phenotypes in A-C as a function of the molar
ratio of mRNAs injected. Note that the incidence of each phenotype depends on
the relative dose of Dlx3 FL mRNA injected.