Fig. 7. C3G mutant MEFs showed impaired cell adhesion and spreading and enhanced cell migration. (A,B) Cells were plated in serum-free medium onto substrates as indicated. (A) One hour after plating, non-adherent cells were removed by washing. Attached cells were stained and counted by photometric absorption. C3G mutant MEFs showed a 66% reduction in adhesion to laminin (^*, P<0.03) and a 23% reduction in adhesion to gelatine (^**, P<0.001). Adhesion to fibronectin appeared normal. (B) Forty-eight hours after plating, few C3G mutant cells remained attached to laminin and gelatine and no cell spreading had occurred. Lack of spreading on fibronectin was also evident. Bar represents 185 µm in all panels. (C) Monolayer wounding assay in serum-containing medium on tissue culture plastic. Lower panel immediately after wounding, upper panel 7 hours later. The mutant cells covered 20% more surface area than the controls in 7 hours Bar represents 400 µm in all panels (0.59 mm²±0.03 vs 0.49 mm²±0.02, ^*, P<0.02, along 4.4 mm wound edge). (D) Graphic representation of the cell migration assays.

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