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Fig. 3. Migrating GnRH-neurons express Ebf2. Arrowheads in A-C indicate cell bodies
of migrating neurons. (A) GnRH-like immunostaining in
Ebf2-/- neurons migrating dorsally through the nasal
mesenchyme at embryonic day 15. (B) Anti ß-galactosidase staining in the
same neurons. (C) Superimposed signals from A and B revealed co-expression of
Ebf2 and GnRH in migrating neurons. (D) Hoechst nuclear counterstaining of
same field. As described (Wray,
2001), GnRH-neurons migrate through cell-poor, nerve fiber-rich
regions. Scale bar: 20 µm.
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