Fig. 6. Ebf2 is expressed in embryonic, postnatal glia and in postnatal motoneurons. (A) E12.5 DRG neurons positive for the heavy chain neurofilament (NF-H) are negative for lacZ staining (Ebf2); however, lacZ staining is observed in presumptive satellite cells adjacent to NF-H positive neurons. (B) In E12.5 dorsal roots, NF-H-like staining corresponding to DRG neuron axons does not colocalize with lacZ staining. (C) In E16.5, peripheral glial cell precursors are positive for p75 NGFR and lacZ. (D) In semi-thin transverse sections of P15 null mutant sciatic nerve, Ebf2 (lacZ staining) is expressed in both myelin forming (msc, arrow) and non-myelin forming (nmsc, arrowhead) Schwann cells. (E) Electron microscopy (EM) image of a heterozygous P15 sciatic nerve stained with bluo-gal, revealing Ebf2 expression (arrows) in nmsc. (F) EM image of a P15-null mutant nerve stained with bluo-gal, revealing persistent expression of Ebf2 in mutant msc (arrows). (G) Low-magnification image of a vibratome transverse section of an heterozygous P30 spinal cord, at the level of the lumbar swelling, revealing Ebf2 expression in dorsal (2,3), commissural (4,10) and ventral (9) laminae of the gray matter. H corresponds to the boxed area in G and reveals Ebf2 colocalization (arrows) with the motoneuron marker choline acetyltransferase (ChAT). 2-9, spinal cord layers 2-9; cs, corticospinal tract; gr, fasciculus gracilis. Scale bars: 10 µm in A,C; 5 µm in B; 10 µm in D; 2 µm in E; 1 µm in F; 80 µm in H.

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