Fig. 1. nti mutation gives similar phenotypes to those defective in Notch signalling. (A-D) nti is a maternal neurogenic gene. Embryos were stained with anti-Elav to detect all differentiated neurons (Robinow and White, 1988). (A) Wild-type embryo shows normal nervous systems. (B) nti mutant embryos derived from heterozygous mothers also have a normal embryonic nervous system. (C) Embryos that lack both maternal and zygotic nti function show a severe neurohyperplasia, and their germ band fails to shorten. (D) Paternally supplied nti gene could hardly rescue the maternal effect neurogenic phenotype; however, germ band retraction occurred in these embryos. (E) Wild-type wing. (F) nti mutant clones are associated with wing nicking and vein thickening (arrowhead). Both are typical phenotypes associated with defects in Notch signalling. (G) Wg is expressed at the DV boundary in wing imaginal disc. (H) Wg expression (purple) is lost in nti mutant clone (arrowhead). nti clones are detected by lack of GFP expression (green). (A-D, G-H) Anterior is towards the left and dorsal is upwards.

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