Fig. 4. Genomic structures of Pphb7 in wild type and transformants and northern analysis of Pphb7. Genomic structure of Pphb7 in wild type (A), Pphb7-GUS (B), GFP-Pphb7 (C) and Pphb7 disruptant (D). The boxes and the lines between the boxes indicate the exons and introns. The circle and square indicate putative start and stop codons. The gray and black boxes indicate a homeodomain and a leucine-zipper motif. The uidA, sGFP, nos-ter, and nptII designate the uidA-coding region, GFP-coding region, nopaline synthase polyadenylation signal, and NPTII expression cassette, respectively. Each bracket above the genome structure indicates the region contained in the plasmid used for gene targeting. P, PmaCI;, S, SalI; X, XbaI; Ba, BamHI; Bg, BglII; E, EcoT14I. (E) Northern analysis of Pphb7. Poly(A)⁺ RNA (1.0 µg) from protonemata (P) and gametophores with rhizoids (G) of wild type was hybridized with cPphb7 probe in (A). As an internal control, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a probe. (F-H) Southern analyses to confirm gene targeting. Genomic DNA of wild type (wt) and transformants was digested with BglII (F,H) or EcoT14I (G), and the gPphb7 probe was used. (F) Pphb7-GUS-1 and -2 lines (lanes 1,2); (G) GFP-Pphb7-1 to -10 lines (lanes 1-10); (H) Pphb7dis-1 to -5 lines (lanes 1-5).

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