Fig. 8. Rhizoids of the wild-type and Pphb7 disruptant lines. (A) Gametophores with 12 leaves in the wild type (left), Pphb7dis-2 (middle), and Pphb7dis-3 (right). (B-D) A Pphb7dis-3 gametophore with 18 leaves was serially sectioned at the position of the leaf above the uppermost mid-stem rhizoid (B), where the leaf merges to a stem (C), and where the uppermost mid-stem rhizoid (asterisk) emerges (D). The abaxial leaf epidermal cells (asterisk in B) and the stem epidermal cells (asterisk in C) in the same longitudinal cell file as the uppermost mid-stem rhizoid. Arrows in C indicate leaf traces. Gametophores of the Pphb7dis-3 grown for 6 weeks in the presence of 0.1 (E), 1.0 (F) and 10 (G) µM NAA. (H,I) Merged images of chlorophyll autofluorescence by UV excitation and SYBR Green I staining of chloroplasts in the fifth cell from the rhizoid apical cell of wild type (H) and Pphb7dis-3 (I). The yellow spots are chloroplast nucleoids. (J,K) Transmission electron micrographs of rhizoid chloroplasts from wild type (J) and Pphb7dis-3 (K). Scale bars: in A, 1 mm for A; in E, 1 mm for E-G; in B, 100 µm for B-D; in H, 2.5 µm for H,I; in J, 0.5 µm for J,K.

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