DEV00717 Supplemental Data

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• Figure S1 - Ventral telencephalic expression of Shh in Nkx2.1 and Pax6 mutants. As revealed by immunostaining of coronal telencephalic sections taken from 22 and 24 somite embryos, Shh (red) is normally expressed nested within the Nkx2.1-positive domain (green) in the subpallium (A). Expression of Shh is lost at early stages in the subpallium of Nkx2.1^–/– mutant mice (B). Note the Nkx2.1 antibody still recognizes a very low level of mutant protein in the Nkx2.1^–/– mutant subpallium (faint green). Expression of Shh in the subpallium is unaffected in Sey/Sey mutant mice lacking Pax6, as shown in coronal sections from stage 22-23 somite embryos (C,D). Scale bar: 50 mm (A-D).