Fig. 1. Expression of patterning genes between E9.5 and E10.5. (A) As shown in coronal sections, at 23 somites (E9.5), expression of Pax6 (green) and Nkx2.1 (red) forms a boundary in the ventral telencephalon (arrow). (B) Higher power view shows Shh (blue) expression in the ventral-most telencephalon. (C) The border of Pax6 and Nkx2.1 expression, and nested Shh expression, is also shown. (D) By 27 somites (E10.0), a slight gap (arrows) appears between Pax6 (green) and Nkx2.1 (red) expression; (E,F) Gsh2 is (blue) expressed laterally (arrows in E). (G) By E10.5, the gap in the expression of Pax6 (green) and Nkx2.1 (red) expands (arrow). (H,I) Gsh2 (blue) is expressed in this gap (I) and is partially overlapping (white) with Pax6 expression (green; arrow; H). (I) At this stage, expression of Gsh2 (blue) is also no longer restricted to the lateral domain, but expands more ventrally into the Nkx2.1-positive (red) domain. Ctx, cerebral cortex; LGE, presumptive future lateral ganglionic eminence; MGE, presumptive future medial ganglionic eminence. Scale bars: 50 µm (A,D,E,F); 25 µm (B,C).

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