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Fig. 4. Forebrain defects in GPC4-depleted embryos. (A-D) Embryos injected with
CoMo. (E-H) Embryos injected with Gpc4Mo. (A,E) Frontal view of Sox2
distribution at stage 21. White arrowheads indicate the level of the
transverse sections shown in panels B-D and F-H. (B-D,F-H) Histological
sections are at the level of the forebrain in panels B and F, the midbrain in
panels C and G, and the spinal cord in panels D and H. Arrowhead in panels F
and G points to defects in dorsal neural tube closure. No, notochord; So,
somites.
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