Fig. 3. Peripodial and disc proper cells are clonally related. Actin5C>lacZ clones were induced by Ubx-GAL4/UAS FLP. All discs were stained with anti-ß-galactosidase antibodies. (A) A wing disc showing clones in both peripodial membrane and disc proper. After confocal imaging, the optical sections were reconstructed using 3D imaging software, which would distinctly show both large peripodial and small disc proper cells. (B-D) Optical sections of a part of the same wing disc showing PM cells (B) and DP cells (C) at higher magnification. The optical section in C focuses on only a few DP cells in the field. The two optical sections were differently colour-coded and merged together to show spatial relationship of PM (green) and DP (red) clones (D). (E-H) Peripodial-only clones. Size of peripodial-only clones varied from just two cells (E) to >128 cells (H). In F-H, there appear to be more than one clone, as medial edge cells are also stained. We did not observe any disc with clone/s only in the disc proper. (I-K) Haltere discs showing both PM and DP clones. In some discs, the relative size of DP clones is much larger than those in wing discs (I-J). For example, in I, DP clone/s cover the entire anterior compartment.

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