DEV00713 Movies

Files in this Data Supplement:

• Movie 1 - Timelapse movie showing how axons establish the POC. Time interval between each frame is 3 minutes.
• Movie 2 - Timelapse movie showing axons growing and establishing the POC commissure. The initial axon projecting towards the midline was injured immediately before crossing it by heat. Over the next few frames, blebbing caused by heat can be seen as the axon deteriorates. Later, follower axons can be seen in subsequent frames of the movie. Only the first follower axon slows down in the midline domain following the injury of the original leader axon. The commissure eventually forms correctly.
• Movie 3 - Timelapse movie showing another POC tract being established. Initially, two growth cones, one from each of the vrc, project toward the midline with one crossing the midline region first. Although, initially, both growth cones have complex morphologies, upon contacting each other they change shape and become elongated, even though the second growth cone has not yet crossed the midline. Midline kinetics of the second axon are similar to later follower axons.