Fig. 3. Effects of glo1-1 on embryo and endosperm morphology. Transition-stage embryo in (A) wild-type and (B) glo1-1, showing small, dense cytoplasmic cells (circle) located in the defective suspensor (s). Endosperms (8 dap) in (C,E,G) wild-type and (F,H-J) glo1-1. (C) Wild-type basal endosperm transfer layer (BETL; betl in figure). (D) Two large cells (designated with arrowhead) interrupt the arrangement of the glo1-1 BETL. (E) Wild-type starchy endosperm (SE; se in figure). (F) Disorganised SE in glo1-1 often containing clusters of small cells and enlarged cells. (G) Wild-type aleurone (al). (H,J) Aleurone development in glo1-1: (H) two aleurone layers, (I) abnormal proliferation of aleurone in the glo1-1 apical endosperm and (J) lack of a regular aleurone layer. Additionally, cell wall stubs (arrows) and multinucleate cells (asterisks) were observed in glo1-1 embryos (data not shown) and endosperms (D,F,I,J). Circles highlight areas of interest. e, embryo-proper; esr, embryo surrounding region; pc, placento-chalazal region. Scale bars: 50 µm in A-D,G,H,J; 100 µm in E,F,I.

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