spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Inhibition of {gamma}-secretase in cultured metanephroi eliminates Notch activation and reduces tissue complexity. (A,E) Activated Notch1, detected with an antibody against the antigen generated by {gamma}-secretase (VLLS) (Schroeter et al., 1998), is present in the nucleus of S- and comma-shaped bodies (red circles) in 14.5 dpc embryonic kidney. (E) Control with no primary antibody. (B-D) Metanephroi cultured in DMSO-containing media or (F-H) 1 µM DAPT-containing media. NICD is present in control (B) but is undetectable after 5 days in DAPT (F). After the first day of culture, phase contrast images of control (C) and DAPT-treated metanephroi (G) are indistinguishable. After 5 days, the control metanephros is filled with high contrast structures (D), while the DAPT-treated contralateral metanephros has branches but less tissue complexity (H).





Right arrow Return to article