Fig. 2. Whole-mount images of cultured 12.5 dpc metanephroi. Top two rows, DBA stain (ureteric bud/collecting ducts). Bottom two rows, Wt1 stain. In both cases representative metanephroi are shown. The treatment and time in culture are indicated in the figure. With DAPT, less extensive branching is evident after 3 days in culture. Ducts appear dilated, and the epithelium at the tip of a few end branches formed bubble-like structures (day 5, asterisk and insets). Wt1 staining reveals fewer Wt1^HIGH structures (white arrows) and more Wt1^LOW cells (black arrows) in DAPT-treated metanephroi from day 2 onwards. Scale bar: 0.5 mm.

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