Fig. 8. (A,B) Proximal epithelia can form in the absence of podocytes after DAPT removal. The section shown in Fig. 7L is shown here (A) next to an adjacent section stained with laminin 1 (green) and E-cadherin (blue; B). The sections are aligned to the same orientation (ducts are marked with blue asterisks). NICD-positive (pink) cells are surrounded by laminin 1 and are E-cadherin^LOW (yellow asterisks). In addition to these proximal epithelial, laminin 1 and E-cadherin^HIGH distal epithelia are also detected. Ducts did not stain with laminin 1 in this experiment. (C) To confirm the proximal tubule identity, another section from the same metanephros was stained for cadherin 6. (D) Contralateral metanephros cultured continuously in DAPT contained no cadherin 6-positive cells. Bars indicate days with DAPT (blue) or DMSO (white).

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