Fig. 4. Cell transplantation experiments between wild-type and vgo/tbx1 larvae (tm208 allele). (A,B) Ventral views of 3 dpf vgo/tbx1 larvae stained with Alcian Blue, showing the rescue of cartilages (arrows) in the vicinity of transplanted Tar*-expressing wild-type cells (black staining). (A) p6 is rescued only on the left side of the embryo and is absent on the contralateral side. (B) m and ch cartilages are rescued on the left side, judged by their shape and cell number compared with the contralateral side. (C) Lateral view of left ear of a 3 dpf vgo/tbx1 larva. Transplanted wild-type cells (in red fluorescence) contributed to the developing semicircular canals (arrows). (D,E) The same larva as in C at 5 dpf. Wild-type cells were able to partially restore ear size and the presence of semicircular canals (arrow) in the left ear (E), whereas the right ear, which did not receive any wild-type cells, remained small (D) and without semicircular canals. cb1-5, ceratobranchial cartilages 1-5; ch, ceratohyal cartilage; hm, hyomandibula; m, Meckel's cartilage of mandibular arch; nc, notochord; oa, occipital arch; pq, palatoquadrate; pc, parachordalia; t, trabeculae.

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