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Fig. 5. Expression of vgo/tbx1 pathway genes in mutants. Ventral views
with anterior towards the left in all panels. (A,B) Expression of
edn1 in wild-type (A) and vgo/tbx1 (B) 27 hpf embryos. (C,D)
Expression of tbx1 in 25 hpf wild-type (C) and vgo/tbx1 (D)
embryos. In vgo/tbx1 embryos, tbx1 mRNA is still present in
cells ventral to the otic vesicle (Fig. 5D, arrows). At this stage, the otic
vesicle in vgo embryos no longer express tbx1. (E,F)
edn1 expression in 25 hpf wild-type (E) and vgo/tbx1 (F)
embryos. In vgo/tbx1 embryos, edn1 expression is very much
reduced in cells immediately anterior to the otic vesicle. The downregulation
of edn1 in the arches is not due to the absence of cells, as
tbx1-expressing cells are present (D). (G,H) Expression of
hand2 in 34 hpf wild-type (G) and vgo/tbx1 (H) embryos.
(I,J) Expression of crestin in 20-somite wild-type (I) and
vgo/tbx1 (J) embryos. Absence of hand2 expression in the
posterior arches of vgo/tbx1 embryos is not caused by the absence of
neural crest cells, as revealed by the pan-neural crest marker
crestin in vgo/tbx1 embryos (J). Arrows indicate neural
crest cells ventral and posterior to the otic vesicle.
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