Fig. 2. RNA expression patterns of Robos and Slits in the AOB. In situ hybridisation experiments were performed on sagittal sections of the AOB at P1. The outline of the entire AOB is given by broken lines in A. VNO axons in the nerve layer (n) form synapses with mitral- and tufted cells (m/t) located ventrally in the AOB. (A,B) M/t cells along the entire anteroposterior axis of the AOB express Robo1 (A) and Robo2 (B) RNA. Robo2 is also strongly expressed in m/t cells of the MOB (arrows in B). (C) Probing AOB sections with Robo2 sense riboprobes result in no staining. (D,F) Slit1-(D) and Slit3-(F) expressing cells are concentrated at the very anterior border of the AOB (arrows). (E) By contrast, Slit2 RNA is expressed throughout the AP axis of the AOB. AOB, accessory olfactory bulb; A, anterior; D, dorsal; L, lateral; M, medial; MOB, main olfactory bulb; m/t, mitral and tufted cells; n, nerve layer; P, posterior; V, ventral. Scale bar: 100 µm.

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