Fig. 5. Effect of BMP4 and noggin on digit regeneration. (A-C) Exogenous treatment with BMP4 rescues the Msx1 regeneration defect. (A,B) Frontal section in situ hybridization with distal toward the top of the image. (C) Sagittal section in situ hybridization with distal to the top and dorsal to the left of the image. BMP4-induced mutant digit regeneration displays normal expression of Msx2 (A), Bmp4 (B) and Hoxc13 (C). (D-F) Exogenous treatment with the BMP-binding protein noggin inhibits regeneration in wild-type digits. (D-F) Frontal section in situ hybridization with distal toward the top of the image. Despite the absence of a regeneration response, stump tissues maintain expression of Msx1 (D), Msx2 (E) and Bmp4 (F).

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