Fig. 1. Relative timing of EFTF expression. RT-PCR was used to detect the expression of ET, Pax6, Six3, Rx1, tll, Lhx2 and Optx2 in the unfertilised embryo (E) and until stage 18 of development. The transient expression of Six3 and tll prior to stage 10.5 was detected in four independent experiments. PCR amplification of Histone H4 demonstrates that approximately equivalent amounts of cDNA templates were used. A duplicate set of reactions from stage 18 embryo RNA were run without reverse transcriptase to test for contaminating plasmid and genomic DNA (18-RT). The PCR products were subcloned and sequenced to confirm their identities. (B) Schematic showing the results of multiple experiments. Each dot represents the developmental stage at which strong induction was observed.

Return to article