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Fig. 2. DOC1R mRNA and protein are present in mouse oocyte. (A) Expression of DOC1R mRNA in immature oocytes (lanes 1 and 2) and ovaries (lane 3), treated or not with reverse transcriptase (RT, + or -). (B) The anti-DOC1R antibody recognizes a band at 28 kDa in 200 oocytes arrested in metaphase II (lane 2) and recognizes 1 µg of purified DOC1R protein (lane 4). The antibody pre-incubated with the DOC1R peptide (peptide, + or -) does not recognize either endogenous DOC1R (lane 1) or purified DOC1R (lane 3). These experiments were repeated twice.





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