Fig. 3. Formation of mesenchyme detected with Mch-3 antibody in macho-1-deficient and -overexpressing embryos. (A,B) Expression of mesenchyme-specific Mch-3 antigen. (A) Control larva in which control MO was injected. Anterior is towards the left. (B) 100 pg of macho-1 MO was injected. (C) Diagram illustrating the vegetal hemisphere of the 110-cell stage embryo. Presumptive mesenchyme blastomeres are indicated in green. (D-G) Expression of mesenchyme-specific Mch-3 antigen. Embryos whose cleavages were arrested at the 110-cell stage. (D) Control embryo injected with control MO. Anterior is upwards. (E) 100 pg of macho-1 MO was injected. (F) PVC-removed embryo. (G) 50 pg of macho-1 mRNA was injected. White arrowheads indicate ectopic expression of Mch-3 antigen in the anterior half. Anterior is upwards. (H) Lateral view of the eight-cell stage embryo and fate map. Nerve cord (NC), notochord (Not), endoderm (En), mesenchyme (Mes) and muscle (Mus)-forming areas are indicated. Color of each area is the same as in Fig. 1. (I) Expression of mesenchyme-specific Mch-3 antigen in the partial embryos derived from isolated blastomere of the eight-cell embryos. Embryos were injected with 50 pg of control macho-1 mRNA that lacks the zinc-finger domain or 50 pg of macho-1 mRNA. After isolation, blastomeres were treated with FGF or BSA only. Scale bars: 100 µm.

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