Fig. 4. Expression of notochord markers in macho-1-deficient and - overexpressing embryos. (A-D) Expression of HrBra gene at the 110-cell stage. (E-H,G',H') Expression of notochord-specific Not1 antigen in embryos whose cleavage was arrested at the 110-cell stage. (A,E) Embryos injected with control MO. Anterior is upwards. (B,F) PVC-removed embryos. (C,G,G') Embryos injected with (C,G) 100 pg and (G') 300 pg of macho-1 MO. White arrowheads indicate ectopic expression in mesenchyme precursors. Anterior is upwards. (D,H,H') Embryos injected with (D,H') 100 pg and (H) 50 pg of macho-1 mRNA. (I) The vegetal hemisphere of the 110-cell stage embryo. Color of blastomeres is the same as in Fig. 1. (J M) Expression of Not1 antigen in the partial embryos derived from isolated mesenchyme precursor blastomeres. (J,K) B8.5 partial embryos. (L,M) B7.7 partial embryos. (J,L) Isolates from control embryos. (K,L) Isolates from embryos injected with 100 pg of macho-1 MO. Scale bars: 100 µm.

Return to article