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Fig. 7. Two-step model of binary fate specification in the marginal cells of the vegetal hemisphere in ascidian embryos. (A) There are two distinct steps to specify the four cell types. The first step is inheritance (or not) of macho-1. The second step is receipt (or not) of the FGF signal. (B) Presence or absence of macho-1 is responsible for making cell responses different in mesenchyme and notochord induction. The molecular identity of the PVC factor shown in Fig. 1D is the macho-1 product.





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