Fig. 1. Expression of Hox and non-Hox genes among motoneuron subtypes in the central hindbrain. (A) Lateral view of an E11 embryo stained with TuJ1 showing the region of the central hindbrain labeled by 4, 5 and 6 to indicate the individual rhombomeres examined in this study. (B) Flat-mount preparation of an E11.5 embryo containing a targeted Hoxb1^GFP reporter (white). Hoxb1^GFP labels the progenitors and differentiating branchiomotoneurons (BMN) in r4 and their migratory course (arrow) towards the ventrolateral region of r6. Two classes of motoneurons, facial BMNs (green) and abducens somatic motoneurons (SMNs, red), are represented by spheres superimposed over the expression of Hoxb1^GFP to illustrate their spatial relationship. (C-E) Transverse sections of E11.5 embryo spanning r4, r5 and r6 labeled with Hoxb1^GFP (green), Phox2b (blue) and Isl1/2 (red). (F-H) E11.5 hindbrain flat-mount RNA in situ hybridization for Hoxa3, Hoxb3 and Hoxd3. V, Ventral; D, Dorsal.

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