Fig. 3. Misexpressed CBF1 induces expression of ephrin A5 and ephrin A2, and repressed that of EphA3 and CBF2. (A,B,F,G,K,L,P,Q) Horizontal section in situ hybridization of control retinae (A,F,K,P) and CBF1/RCAS-electroporated reginae (B,G,L,Q) of E8 embryos. (D,E,I,J,N,O,S,T) Respective enlargements of the boxed temporal areas. Sections were hybridized with antisense probes for EphA3 (A,B,D,E), ephrin A5 (F,G,I,J), ephrin A2 (K,L,N,O), or CBF2 (P,Q,S,T). Nasal (anterior) is upwards, temporal (posterior) is downwards. Scale bar: 600 µm. (C,H,M,R,U) Northern blot analysis of E8 chick retina electroporated with CBF1/RCAS. Northern blot analysis was performed using 20 µg of total RNA prepared from the nasal (N) and temporal (T) thirds of E8 retinae transfected with CBF1/RCAS (CBF1). RNA of control retinae (control) was prepared from the left eyes of the same embryos. Probes used for each panel are indicated on the right. A probe for GAPDH, glyceraldehyde phosphate dehydrogenase, was used to indicate the amount of RNA present.

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