Fig. 1. HOB gene expression in wild-type and mutant males. Left lateral views (anterior leftwards, ventral downwards). Scale bar: 20 µm. (A1,A2) Expression of ceh-26::gfp in the HOB neuron of a wild-type adult male. Absence of fluorescence in an egl-46(sy628) mutant (A3), an egl-44(n1080) mutant (A4) and a daf-19(m86) mutant (A5). (B1,B2) HOB and ray expression of pkd-2::gfp was observed in a wild-type adult male. (B3) An egl-46(sy628) male with ray but not HOB expression. (B4) An egl-44(n1080) male with expression in both HOB and ray cells. (B5) No visible expression in both HOB and rays of a daf-19 mutant. (C1,C2) Normal osm-6::gfp expression in HOA and HOB at the L4 stage. Expression was not affected in egl-46(sy628) (C3) and egl-44(n1080) mutants (C4). (C5) No expression was observed in HOA and HOB cells of a daf-19(m86) mutant male. Cell positions of HOB in A3,A4,A5,B3,B5, and HOA and HOB in C5 were located by overlaying with the Nomarski pictures of the same animal. Hook structure autofluorescence is indicated by small arrows. The original osm-6::gfp strain has a ncl-1(-) background. ncl-1(-) was still present in the him-5 strain of osm-6::gfp integrant (C1,C2) and an egl-46(sy628) mutant background (C3), but was crossed out in egl-44(n1080) (C4) and daf-19(m86) mutants (C5). egl-46(sy628) mutant also had a dpy-11 mutation in the background (C3). No effect on osm-6::gfp expression was detected for ncl-1(-) and dpy-11 mutations.

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