Fig. 3. Examination of tail wholemounts at various stages of the hair growth cycle. Wild-type mice were injected with BrdU according to the LRC protocol and wholemounts were prepared from mice of the following ages: 28 days (early anagen; A,B,E,H,K), 35 days (anagen; C,F,I,L) and 42 days (telogen; D,G,J,M). (A-D) Double labelling for BrdU (green) and Ki67 (red). Arrows indicate representative follicles in early anagen (A), full anagen (C) and telogen (D). Arrowheads in B indicate high-magnification examples of double-labelled cells in early anagen. (E-G) Double labelling for TUNEL positive cells (green) and keratin 14 (red). Arrows indicate TUNEL-positive cells. Arrowheads indicate non-specific staining of hair shafts. Asterisk indicates a follicle in full anagen. (H-J) Immunolabelling for CDP. Arrows indicate representative follicles in early anagen (H), full anagen (I) and telogen (J). (K-M) Immunolocalisation of 6 integrin. Brackets indicate regions of the hair follicle with strong 6 integrin labelling during early anagen (K), anagen (L) and telogen (M). Scale bars: 100 µm in A,C-M; 20 µm in B.

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