Fig. 4. Loss of LRC following treatment with TPA. Wild-type mice were injected with BrdU according to the LRC protocol. After a chase period of 10 months, wholemounts of tail epidermis were collected from untreated mice (A) or after thrice-weekly treatment with TPA (B-D,F,H,I) or acetone (E,G,J) for the number of days indicated. Wholemounts were labelled to detect Ki67- (red, AG), BrdU- (green, A-G), keratin 14- (red, H-J) or TUNEL- positive cells (green, H-J). (A-G) Asterisks indicate hair follicles that contain very few LRC. (F,G) Arrowheads in high magnification micrographs indicate double-labelled cells. (H-J) Arrows indicate TUNEL-positive cells. Asterisks indicate non-specific staining of hair shafts. BG, bulge; SG, sebaceous gland. Scale bars: 100 µm in A-E,H-J; 20 µm in F,G.

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