Fig. 7. Effects of NLef1 transgene on tail epidermis. Tissue sections (A-D) and wholemounts (E-P) of wild-type (A,C,E,G,I,K,M,O) and K14NLef1 transgenic mice (B,D,F,H,J,L,N,P) at 3.5 weeks (A,B.E,F,I,J,M,N) and 3.5 months (C,D,G,H,K,L,O,P) of age. (A-D) Hematoxylin and Eosin staining. Note epithelial cysts (EC) at the base of hair follicles (HF) in transgenic skin. IFE, interfollicular epidermis; SG, sebaceous glands. (E-H) Wholemounts immunolabelled for keratin 14 (red) and LRC (green). Arrows in F indicate bending of base of hair follicles associated with early cyst formation. Arrows in H show loss of LRC in deformed hair follicles of transgenic mice. (I-L) Staining with Nile Red. (I,K) Staining restricted to sebaceous glands (SG). (J,L) Arrows indicate increased sebocyte differentiation along the length of hair follicles. (M-P) Immunolabelling for ß1 integrins (red) and CDP (green). Immunolabelling for CDP is observed in wild-type anagen follicles (arrows in M,O) and some follicles of 3.5-week-old transgenic mice (arrows in N), but is completely lost from the abnormal follicles of older animals (arrowheads in P). Scale bar: 100 µm.

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