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Fig. 5. Erbb4Flox/FloxWap-Cre mice exhibit defects in mammary epithelial proliferation. Immunohistochemical detection of in situ BrdU incorporation in biparous Erbb4+/+Wap-Cre control (A) and Erbb4Flox/FloxWap-Cre (B) mice at L1. Arrowheads indicate BrdU-labeled nuclei. (C) A significant reduction in the percentage of BrdU-labeled cells was observed in Erbb4Flox/FloxWap-Cre mammary glands at P13 (t[8]=4.41, P<0.01) and L1 (t[8]=9.11, P<0.001), but not at P18 (t[13]=1.71, P<0.20). Significant differences within each data set are represented by asterisks. Scale bar: 50 µm.





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