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Fig. 6. Erbb4Flox/FloxWap-Cre secretory epithelium fails to express the differentiation marker NPT2B. Immunohistochemical staining of NKCC1 (red) and SMA (green) in mammary glands of biparous Erbb4+/+Wap-Cre and Erbb4Flox/FloxWap-Cre mice, at P13 (A,B), P18 (C,D) and L1 (E,F). Immunohistochemical staining of NPT2B (red) and ß-catenin (green) in mammary glands of biparous Erbb4+/+Wap-Cre and Erbb4Flox/FloxWap-Cre mice, at P13 (G,H), P18 (I,J) and L1 (K,L). Lack of NPT2B staining in Erbb4Flox/FloxWap-Cre mammary glands at L1 indicates a defect in epithelial differentiation. Arrowheads (A,B,E) indicate NKCC1 staining and arrowheads (I,K) indicate NPT2B staining of the apical surface of secretory epithelium.





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